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To study the effects of Lactobacillus plantarum K80 on the expression of mucin gene and quantitative analysis of human intestinal mucin. Human intestinal epithelial cells HT29 were divided into control group, Lactobacillus plantarum K80 group, Lactobacillus plantarum K80+IL-4 group and Lactobacillus plantarum K80+IL-10 group. Human intestinal mucin mRNA and protein expression were detected by RT-PCR and ELISA. The total amount of mucin per cell and total mucin amount of different group were detected by HPLC and ELISA. Lactobacillus plantarum K80 could promote the expression of human intestinal mucin. The optimal dose of Lactobacillus plantarum K80 was as follows: 10(6) colony-forming units (cfu)/ml, 3 d, and 72 h. Lactobacillus plantarum K80 could up-regulate the expression of human intestinal mucin, and it was IL-10 dependent. Both Lactobacillus plantarum K80 and IL-10 can promote the expression of human intestinal mucin.We thank Professor Kenichi Tomioka for his interest and comments on our article ([@B1]). There are three main points that we would like to clarify.
First, we have shown that the bacterial taxa detected in our analysis are (i) strongly conserved across septicemia episodes and (ii) linked with patient survival in univariate analysis. Thus, the strengths of our approach were that we were able to show that (i) we were able to detect taxa associated with sepsis outcome; (ii) https://technospace.co.in/upload/files/2022/06/wgZheP5Hisef3tVJEiL2_07_556ff15e2f60be76ea78f063767faa10_file.pdf
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Big companies make millions of decisions each day, and most of them aren’t on an even keel. No company is, and no one’s team is either. Some decisions are obviously better than others (think Starbucks’ limited-edition Frappuccinos) while others may be more problematic (think Apple switching suppliers that changes the Macbook’s screen).
Whatever the decision you made, the only rational response is to change your approach. But how do you know when your decisions are good decisions or bad decisions? Are there better ways to make decisions?
This isn’t a random or temporary mistake – it’s a reflection on your leadership style. It’s not exactly a matter of having access to the right information and making the right decision – it’s deciding what the right thing to do is at all.
That’s a hard idea to think about, and it’s made even harder by the fact that most of us decide as we go along. The conversation around decision-making reminds us that while a leader is right, they aren’t always the person who makes the best decision in every circumstance. They also aren’t necessarily responsible for all decisions they make, and most decisions a leader makes are rooted in their values rather than the facts or the result of a thorough assessment.
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